CYP REACTION PHENOTYPING

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Using recombinant enzymes, one can identify the enzyme responsible for the metabolism of the compounds. This helps to predict and understand potential drug-drug interactions.

CYP Reaction Phenotyping Assay Protocol:

Test article concentration: 5  µM (other upon request) 

Incubation time: 0, 5, 15, 30 and 60 minutes 

Incubation temperature: 37 C 

Number of Replicates: n = 2  

Assay condition: Phosphate buffer 

Assay matrix: CYP1A2, CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6 and CYP3A4   (other isoforms are available) 

Controls: Known isoform specific substrates 

Analysis Method: LC/MS/MS 

Data Delivery: % Remaining parent compound; Half life