BLOOD - PLASMA PARTITIONING

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Whole blood binding in comparison to plasma protein binding is important in identifying if differential binding to a specific component in the blood occurs and in interpreting pharmacokinetic data. 

Drug binding to erythrocytes may lead to overestimation of blood clearance.

Blood - Plasma Partitioning Assay Protocol:

Method: Whole blood centrifugtion

Test article concentration: 1  µM (other upon request) 

Incubation time: 30 minutes 

Incubation temperature: 37 C 

Number of Replicates: n = 2  

Assay condition: 100% Plasma, whole blood (as per request) 

Species: Human; rat; dog; cynomolgus monkey; guinea pig; mouse (others   upon request) 

Positive controls: Methazolamide (human) Chlorthalidone (rat and mouse) Chloroquine (dog) 

Analysis Method: LC/MS/MS 

Data Delivery: Blood to plasma ratio